western blot抗体洗脱液的配方?

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western blot抗体洗脱液的配方?
western blot抗体洗脱液的配方?

western blot抗体洗脱液的配方?
Stripping by Acidic pH
Applicable to any chemiluminescent substrate system.
Required Equipment and Solutions
Stripping solution:25 mM glycine-HCl,pH 2,1% (w/v) SDS
Phosphate buffered saline (PBS):10 mM sodium phosphate,pH 7.2,0.9% (w/v) NaCl
Shallow tray,large enough to hold the membrane
Procedure
1.Place the blot in stripping solution and agitate for 30 minutes.
2.Place the blot in buffer and agitate for 10 minutes.Repeat with fresh buffer.
3.Proceed to the blocking step for the next round of detection.

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