英语翻译In an attempt to identify the function of specific classIII peroxidases,several authors reported the generation of transgenic plants to study different peroxidase genes.However,in A.thaliana only 9 out of 73 peroxidase genes have been ide

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英语翻译In an attempt to identify the function of specific classIII peroxidases,several authors reported the generation of transgenic plants to study different peroxidase genes.However,in A.thaliana only 9 out of 73 peroxidase genes have been ide
英语翻译
In an attempt to identify the function of specific classIII peroxidases,several authors reported the generation of transgenic plants to study different peroxidase genes.However,in A.thaliana only 9 out of 73 peroxidase genes have been identified by this approach (Table 1).The characterisation of individual peroxidase mutants often gives only unconclusive results [8-12].The in planta role of most peroxidases remains therefore elusive.This situation is mainly linked to two difficulties inherent in peroxidases:i) gene redundancy results in no visible mutant phenotype,and ii) the lack of substrate specificity in vitro,prohibits a determination of which compounds are real in planta substrate.Molecular biology approaches seems to offer a powerful tool to overcome these difficulties [7].Transcriptome analysis for example provides a detailed description of the gene regulation during plant growth and development,in any plant tissue and also in different relevant genotypes.Thus a transcriptomic approach should permit an efficient screen showing which peroxidases are expressed at key developmental stages,but also to identify redundant peroxidases genes putatively involved in the same specific process in all kind of tissues,development stages and genotypes.Nevertheless,not all peroxidase genes are represented on commercially available microarrays.For this reason,in this study we used a home made macroarray dedicated exclusively to the 73 class III peroxidase genes of A.thaliana [13]

英语翻译In an attempt to identify the function of specific classIII peroxidases,several authors reported the generation of transgenic plants to study different peroxidase genes.However,in A.thaliana only 9 out of 73 peroxidase genes have been ide
在试图确定具体classIII过氧化物酶的功能,一些作者报告说,转基因植物研究不同代过氧化物酶的基因.然而,在只有9拟南芥过氧化物酶的基因中,有73个已经确定了这一方针（见表1）.过氧化物酶突变体的个体特征往往只提供unconclusive结果[8-12].在大多数过氧化物酶作用的遗体足底因此elusive.This形势,主要是与两个固有的困难,过氧化物：在我没有明显的突变体）的基因冗余的结果,及ii）在体外特异性底物缺乏,其中一个决心禁止在植物基化合物是真实的.分子生物学的方法似乎提供了一个强大的工具来克服这些困难[7].转录例如分析提供了植物生长和发育过程中的任何植物组织,基因调控详细说明,而且在不同的基因型有关.因此,一个转录的方法应该允许一个有效的屏幕显示哪些过氧化物酶均以在关键的发展阶段,也是鉴定公认多余过氧化物酶在同一具体过程涉及在所有的组织,发展阶段和基因型的一种基因.然而,并非所有的代表过氧化物酶基因在商用芯片.出于这个原因,在此研究中,我们使用了自制的,专门的73个第三类过氧化物酶基因的拟南芥[13]宏阵列

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为了识别功能的具体classIII peroxidases几个作者报道,这一代的转基因植物,研究不同的过氧化物酶的基因。然而,在a .芥只有9个从73过氧化物酶基因已经被鉴别通过这种方法(表1),过氧化物酶的突变个体的特征性unconclusive经常给出的只是结果[8 - 12]。在大部分的角色peroxidases见仍因此难以捉摸的。这种情况主要得力于两方面的困难中固有的peroxidases:我)基因突变的结果是没有可见的冗余和ii)的表型,底物特异性缺乏,禁止在体外测定的化合物是真实的在见板上。分子生物学的方法似乎提供了强大的工具来克服这些困难[7]。Transcriptome分析为例提供了详细的描述,在植物生长的基因调控与发展,在任何植物组织也在不同的相关因素。因此方法应该允许一个高效的转录组peroxidases屏幕上显示的表达在关键的发展阶段,而且识别冗余peroxidases基因参与同一卫星拍摄的具体过程,在各种组织发展阶段与基因型。不过,并不是所有的过氧化物酶基因是在商业上可用的微阵列为代表。因为这个原因,在本研究中,我们使用了一种自制的macroarray专门致力于的III类过氧化物酶的基因,73芥[13]a .

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